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Hematological parameters are a valuable means of estimating the physiological   status of fish and, indirectly, the state of their habitat. In order to use   blood parameters as biomarkers, it is necessary to know their normal values   and the referential intervals for a species. Such investigations are   especially valuable in endemic and endangered fish species. In this study,   parameters of erythrocyte lineage of Delminichthys ghetaldii (Steindachner   1882), an endemic species inhabiting the karst waters of eastern Herzegovina,   were investigated. Fish were caught by nets in the region of Fatničko polje   (Fatnica field) after the water outflow in March 2013, blood was taken by   heart puncture and erythrocyte parameters were determined. Their mean values   were as follows: the number of erythrocyte (RBC) = 1.474 x 1012/l; hemoglobin   concentration (Hb) = 72.50 g/l; packed cell volume (PCV) = 0.398 l/l; mean   corpuscular volume (MCV) = 271.19 fl; mean corpuscular hemoglobin (MCH) =   49.36 pg, and mean corpuscular hemoglobin concentration (MCHC) = 193.16 g/l   eryt. Body mass and morphometric features were also estimated and their mean   values were: body mass = 25.49 g, total and standard body length = 14.13 cm   and 11.98 cm, respectively, Fulton coefficient = 1.45. For the values of all   parameters, the normality of distribution was tested, as well as differences   between mean values of males and females. Pearson’s correlation and multiple   regression analyses between mass, morphometric and hematological parameters   have shown that erythrocyte parameters may influence body mass and of   morphometric characteristics in this species

Hematological characteristics of Delminichthys ghetaldii (Steindachner 1882) inhabiting the karst region of eastern Herzegovina