Onychomycosis: Clinical findings, etiological agents and evaluation of laboratory methods | Zendy

Eleonora Dubljanin | Zendy; Aleksandar Džamić | Zendy; Sanja Mitrović | Zendy; Valentina ArsićArsenijević | Zendy; Ivana Čolović Čalovski | Zendy

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Onychomycosis: Clinical findings, etiological agents and evaluation of laboratory methods

Author(s) -

Eleonora Dubljanin,

Aleksandar Džamić,

Sanja Mitrović,

Valentina ArsićArsenijević,

Ivana Čolović Čalovski

Publication year - 2014

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs1402587d

Subject(s) - trichophyton rubrum , fusarium , agar , dermatophyte , aspergillus , isolation (microbiology) , alternaria , microbiology and biotechnology , trichophyton , agar plate , nail (fastener) , biology , antifungal , botany , bacteria , materials science , genetics , metallurgy

The aim of this study was to identify the etiological agents in patients with suspected onychomycosis, and to carry out comparative testing of individual or combinations of tests: direct microscopy with KOH and Blankophor (BP), culturing on Sabouraud’s dextrose agar (SDA), diluted Sabouraud’s dextrose agar (D-SDA) and dermatophyte test medium (DTM). From 70 nail samples (65 toenails, 5 fingernails), 46 (60.5%) had at least one of five positive tests. Isolation was possible in 41, while in 5 samples the presence of fungi was observed by KOH and/or BP. Dermatophytes were most frequently isolated (80.5%) where Trichophyton rubrum was predominant. Candida spp. was isolated in 9.8%, Aspergillus spp. 4.9%, Alternaria spp. 2.4% and Fusarium spp. 2.4%. Application of BP as an individual test was the most sensitive method. The combination of BP with DTM or D-SDA provides the best sensitivity and allows the identification of fungi to the species/genus level

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