Distribution of Human papilloma virus genotypes in cervical cancer tissues | Zendy

Miodrag Stamenković | Zendy; Aleksandra Knežević | Zendy; Igor Kuzmanović | Zendy; Danijela Karalić | Zendy; Tanja Jovanović | Zendy

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Distribution of Human papilloma virus genotypes in cervical cancer tissues

Author(s) -

Miodrag Stamenković,

Aleksandra Knežević,

Igor Kuzmanović,

Danijela Karalić,

Tanja Jovanović

Publication year - 2014

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs1402573s

Subject(s) - genotyping , cervical cancer , genotype , human papilloma virus , cancer , population , dna extraction , incidence (geometry) , biology , virology , medicine , oncology , polymerase chain reaction , genetics , gene , environmental health , physics , optics

Cervical cancer incidence and mortality rates in Serbia are among the highest in Europe and data on Human papilloma virus (HPV) type distribution are scarce. The aim of this study was to determine the prevalence of HPV types in archival specimens of cervical cancer tissues of women in the Serbian population. A total of 45 paraffin-embedded tissue samples of cervical carcinoma were used in this study. The procedure included deparaffinization of tissue samples, DNA extraction, PCR, gel electrophoresis and HPV genotyping by direct sequencing. HPV was detected in 32 samples (71%). Genotyping revealed the presence of 6 high-risk HPV types 16, 18, 33, 45, 53 and 58, where HPV type 16 was the most prevalent type (73.7%). The results of this study and further studies will provide more detailed information about HPV genotype distribution and may contribute to the formulation of national guidelines for the prevention of cervical cancer. [175073

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