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Bovine parainfluenza 3 virus (PI3) causes respiratory infections in cattle   and sheep with great economic losses in livestock. The aim of this   investigation was to determine the significance of molecular methods in the   identification of isolated strains of PI3 virus. Twenty cattle nasal swabs   were analyzed for the presence of PI3 using the standard virology method of   virus isolation in MBDK cell line and virus neutralization test. The   identification of isolated strains was confirmed by RT-PCR and method of   direct sequencing with primers for PI3 fusion (F) protein gene. PI3 virus was   isolated and identified in four nasal swabs using the standard virology   method and RT-PCR. The analysis of nucleotide sequences of isolated PI3   strains showed high similarity with sequences isolated from cattle in Asia.   Our results showed that molecular methods are very useful in the diagnosis of   PI3 infections as well as for the identification and characterization of PI3   strains in Serbia. [Projekat Ministarstva nauke Republike Srbije, br. 31008 i   br. 175073

The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle