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To establish the presence of West Nile virus (WNV) infection in the animal   population in Serbia after the human WNV outbreak, the presence of anti-WNV   IgG antibodies was examined by commercial ELISA of blood sera samples of 130   horses collected in 2012 from 6 stables and 1 settlement in Vojvodina   Province, northern Serbia. During the blood sampling, hibernating mosquitoes   in the vicinity of the sampled horses were collected (31 pools from 4   locations) and tested for WNV presence by real-time RT-PCR. The presence of   anti-WNV antibodies was observed in 49.23% (64/130) horses. Per stable, the   percent of seropositive animals ranged from 35% to 64%. All 31 analyzed pools   of hibernating mosquitoes tested negative for WNV RNA. The WNV-antibody   prevalence of 49.23% obtained in horses during 2012 was much higher than the   prevalence (12%) found in horses during 2009/2010. These results, including   the confirmed seroconversion in eight horses that tested negative in 2010,   indicated an intensive WNV circulation during 2012 in Serbia, and the   necessity of implementing surveillance programs. [Projekat Ministarstva nauke   Republike Srbije, br. TR31084 and III43007

Serological study on WNV presence in horses in Vojvodina after the human outbreak in Serbia in 2012