Open AccessPreliminary study of steep pulse irreversible electroporation technology in human large cell lung cancer cell lines L9981
Author(s) -
Zuoqing Song,
Zhenhua Pan,
Xiaohong Xu,
Weiqiang Wang,
Lingling Zu,
Jing Wang,
Zhitao Song,
Hongyu Liu,
Zhigang Li,
Chengxiang Li,
Jun Chen,
Chenguo Yao,
Qinghua Zhou
Publication year - 2013
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs1303211s
Subject(s) - electroporation , pulse (music) , lung cancer , cell , cell culture , irreversible electroporation , cell growth , materials science , mtt assay , cancer research , biomedical engineering , chemistry , medicine , biology , pathology , voltage , physics , biochemistry , genetics , quantum mechanics , gene
Our aim was to validate the effectiveness of steep pulse irreversible electroporation technology in human large cell lung cancer cells and to screen the optimal treatment of parameters for human large cell lung cancer cells. Three different sets of steep pulse therapy parameters were applied on the lung cancer cell line L9981. The cell line L9981 inhibition rate and proliferation capacity were detected by Vi-Cell vitality analysis and MTT. Steep pulsed irreversible electroporation technology for large cell lung cancer L9981 presents killing effects with various therapy parameters. The optimal treatment parameters are at a voltage amplitude of 2000V/cm, pulse width of 100μs, pulse frequency of 1 Hz, pulse number 10. With this group of parameters, steep pulse could have the best tumor cell-killing effects
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