A novel buckwheat protein with a beneficial effect in atherosclerosis was purified from Fagopyrum tataricum (L.) Gaertn. | Zendy

Xiaoli Zhou | Zendy; Lin Huang | Zendy; Wen Xia Tang | Zendy; Yiming Zhou | Zendy; Qing Wang | Zendy; Zongjie Li | Zendy

Open Access

A novel buckwheat protein with a beneficial effect in atherosclerosis was purified from Fagopyrum tataricum (L.) Gaertn.

Author(s) -

Xiaoli Zhou,

Lin Huang,

Wen Xia Tang,

Yiming Zhou,

Qing Wang,

Zongjie Li

Publication year - 2013

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs1302767z

Subject(s) - fagopyrum tataricum , sephadex , chemistry , fagopyrum , polygonaceae , in vitro , biochemistry , botany , antioxidant , biology , rutin , enzyme

Buckwheat seeds contain many kinds of functional compounds that are of benefit to patients with cardiovascular disease. In this research, a water-soluble buckwheat protein was isolated and purified through a DEAE-Sepharose anion exchange column and Sephadex G-75 gel chromatography. The isolated buckwheat protein fractions exhibited hypocholesterolemic activity in a HepG2 cell model and demonstrated prominent bile acid salt-binding activity in an in vitro assay. The antioxidative activity of protein fractions with hypolipidemic effects was detected in a free radical scavenging experiment. The buckwheat protein fraction with the most obvious hypolipidemic activity and free radical scavenging activity was named as WSBWP. Its molecular weight was estimated by SDS-PAGE electrophoresis to be 38 kDa. It could become a potential candidate in the treatment of atherosclerosis

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