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Alfalfa (Medicago sativa L.) is an important forage legume in Libya. The  genetic diversity of nine alfalfa domesticated varietal populations was  studied using thirteen RAPD primer combinations. The number of polymorphic  fragments detected per primer combination ranged from 8 to 46 bands with an  average of 24 bands. The number of polymorphic bands detected was from 6  (Atalia population) to 37 (Gabsia population). The lowest genetic distance  was 0.058 and the highest was 0.655. The average genetic distance was  (0.356). The dendrogram based on Ward’s minimum variance clustering method  grouped the nine populations into the two main clusters. The first group  included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The  second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity  of RAPD assays for detection of genetic polymorphisms is confirmed in our  study, and results can be utilized in breeding practice

Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers