Open AccessOptimization and partial characterization of endoglucanase produced by Streptomyces sp. B-PNG23
Author(s) -
Azzeddine Bettache,
Abdelaziz Messis,
Estelle Copinet,
Mouloud Kecha,
Nawel Boucherba,
N. Belhamiche,
Francis Duchiron,
Saı̈d Benallaoua
Publication year - 2013
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs1302549a
Subject(s) - cellulase , bran , enzyme , yeast extract , fermentation , streptomyces , enzyme assay , chemistry , strain (injury) , metal ions in aqueous solution , yeast , biocatalysis , food science , nuclear chemistry , biochemistry , metal , biology , bacteria , organic chemistry , catalysis , reaction mechanism , raw material , anatomy , genetics
Streptomyces sp. B-PNG23 was selected as a promising cellulolytic strain and tested for its ability to produce cellulases from agroindustrial residues. A pH value of 7 and temperature of 28°C were found to be optimal for maximum enzyme production. The highest endoglucanase activity was obtained in a medium comprised of wheat bran (2 g/l), yeast extract (2 g/l), NaCl (2 g/l), NH4Cl (2.5 g/l), and (0.4 g/l) of MgSO4. The enzyme was active at a broad range of pH (5-8) and temperatures (40-70°C). The optimum pH and temperature were 6 and 50°C, respectively. In the presence of metal ions Mn2+, Cu2+ and NH4 + the activity of the enzyme increased significantly. The enzyme retained 50% of its activity after heating at 50°C for 6 h. This enzyme could be considered as a thermotolerant biocatalyst that could be utilized in biotechnological applications
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