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Lyme borreliosis is the most common vector-borne disease in the northern  hemisphere. The agents of Lyme borreliosis are borrelia, bacteria of the  family Spirochaetaceae, which are grouped in Borrelia burgdorferi sensu lato  species complex. Borreliae are fastidious, slow-growing and biochemically  inactive bacteria that need special attention and optimal conditions for  cultivation. The isolation of Borrelia from clinical material and their  cultivation is a time-consuming and demanding procedure. Cultivation lasts  from 9 up to 12 weeks, which is much longer than is necessary to grow most  other human bacterial pathogens. Although B. burgdorferi sensu lato is  susceptible to a wide range of antimicrobial agents in vitro, up to now the  susceptibility of individual Borrelia species to antibiotics is defined only  partially. [Projekat Ministarstva nauke Republike Srbije, br. 175011

Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review