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Gamma-rays of dose of 5 Gy using a 60Co source was applied in a single wheat  cross to study the effect on anther culture (AC) parameters. The female  parent was a 6Ug /6D mono-substitution line of cv. Trakia with resistance to  powdery mildew and leaf rust, while the male parent was cv. Aglika,  characterized as one of the best local varieties with high productivity and  grain quality. Callus induction, plant regeneration, green and albino plants  from the control (normal anther culture, 0 Gy) designated C, were compared  with parameters studied in three other groups, derived from anthers  irradiated with 5 Gy (designated AI), calli irradiated with 5 Gy (designated  CI) and anthers and calli irradiated consecutively with 5+5 Gy (combined  treatment group, designated ACI). Anther culture response of the four F1  group of plants was intermediate between the parents, with a slightly  depressive effect of the dose irradiation in the AI and ACI groups. In  general, the effect of the 5 Gy dose of irradiation on androgenesis was  estimated to be negative for at least one group of haploid plants (AI),  derived from irradiated anthers. Green plants were mostly reduced in the  combined treatment group originating from irradiated calli and anthers

Application of a low dose of gamma rays in wheat androgenesis