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Considering the association of increased fructose intake with the rise in  metabolic syndrome and gender differences in its incidence, this study was  aimed at evaluating and identifying the optimal reference genes for the  relative quantification of gene expression by real-time PCR in the adipose  tissue of female rats exposed to a fructose-enriched diet as an animal model  of metabolic syndrome. The transcription of four commonly used reference  genes, BA, HPRT1, TBP and B2M, was quantified by TaqMan real-time PCR. The  expression stability of these genes was evaluated by direct comparison of Ct  values and by using the geNorm and NormFinder algorithms. NormFinder revealed  HPRT1 as the most stable gene, and both programs identified HPRT1 and TBP as  the most stable combination of genes in this experimental paradigm. In  addition, HPRT1 was shown to be reliable enough as the sole reference gene.  [Projekat Ministarstva nauke Republike Srbije, br. III41009

Selection of reference genes for normalization of real-time PCR data in visceral adipose tissue of female rats on a fructose-enriched diet