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The isolation and molecular characterization of bacterial strains isolated  from water sources in the Vlasina Mountain in southeast Serbia, confirmed the  presence of a new species Chryseobacterium vrystaatense ST1. This Gram-  negative species showed an extremely low level of biochemical reactivity in  biochemical tests. The gene for 16S rRNA was amplified by PCR using universal  primers and sequenced. Comparison of 16S rRNA gene sequence and phenotypic  features indicated that the isolate ST belonged to Chryseobacterium  vrystaatense. A BLAST search of sequenced 1088 nucleotides of the 16S rRNA  gene with all sequences deposited in the NCBI collection showed the highest  similarity (98%) with the strain Chryseobacterium vrystaatense sp. nov.,  designated as strain R-23533. The very high homology of these two strains  allowed classification of our strain at the species level, but some  differences indicate, and indirectly confirm, that the isolate ST is an  authentic representative. On the basis of these results, we could conclude  that Chryseobacterium vrystaatense ST was for first time isolated in Serbia,  which is particularly important when one bears in mind that there are only  three sequences of this species deposited in the NCBI collection

Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia