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The forage legume ochrus chickling [Lathyrus ochrus (L.) D.C.] which is  distributed in the Mediterranean region, is gaining importance in terms of  economy and agriculture in Turkey. However, the full potential of the legume  has yet to be realized due to the presence of neurotoxin, β-N-oxalyl-L-a,  β-diaminopropionic acid (ODAP) causing lathyrism. This study aimed to develop  an efficient micropropagation system using longitudinally sliced cotyledon  node explants for use in Agrobacterium-mediated genetic transformation in the  future. The results show that the maximum number of shoots per explant was  achieved on MS medium solidified with 8 g/l isubgol gelled medium containing  0.30 mg/l BA-0.2 mg/l NAA. Well-developed shoots were rooted by pulse  treatment with 50 mg/l IBA and culturing on an 8 g/l isubgol gel solidified  MS medium. The results showed 60% rooting in the treated shoots. The rooted  plantlets were transferred to pots containing sand and organic matter and  acclimatized

Plant regeneration from pulse-treated longitudinally sliced half cotyledon node explants of Turkish ochrus chickling [Lathyrus ochrus (L.) D.C.]