English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

In this study we report on the effect of glycerol used in storage buffers, on  the inhibitory kinetic behavior of NADP+ on rat kidney glutathione reductase  (rkGR) stored at -80°C in ± 10% (v/v) glycerol. At fixed [GSSG] and varied  [NADPH], rkGR stored ± glycerol were inhibited competitively by NADP+.  Although the addition of glycerol decreased Km (9.7 ± 0.9 μM) and Ki(NADP+)  (26.6 ± 2.2 μM) for NADPH, the catalytic efficiency (kcat/Km = 2.75 x 107  M-1.s-1) was increased 1.64 fold. At fixed [NADPH] and varied [GSSG], rkGR  exhibited noncompetitive (Ki = 397 ± 69 μM) and linear mixed-type (Ki = 59.6  ± 8.4 μM; Ks = 49.9 ± 3.2 μM; α = 7.74) inhibitions with and without glycerol  during storage. The kcat/ Km of rkGR stored in glycerol was 5.16 x 106  M-1.s-1. Although the increase in kcat was 1.25 fold, the kcat/Km was  minimally affected (1.06)

The effect of glycerol in the storage medium on the inhibitory kinetic behavior of glutathione reductase