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Random amplified polymorphic DNA (RAPD) analysis is a simple and reliable  method used to detect DNA polymorphism. Several factors can affect the  amplification profiles, thereby causing false bands and non-reproducibility  of the assay. In this study, we analyzed the effects of different  concentrations of primer, magnesium chloride, template DNA and Taq DNA  polymerase to develop and standardize a RAPD protocol for Centaurium species.  The optimized PCR reaction mixture included: 50 ng of DNA extracted using a  CTbased protocol, 2.5 mM MgCl2, 7.5 pmol primer and 2 U of Taq polymerase  in a final volume of 25 μl. Each of the five primers used in experiments  (OPB11, OPB15, OPB18, OPF05 and OPH02) generated reproducible and  distinguishable fingerprinting patterns of four Centaurium species. The  obtained optimized RAPD protocol and the selected primers are useful for our  further work in the genetic diversity studies of Centaurium species

The reproducibility of RAPD profiles: Effects of PCR components on RAPD analysis of four centaurium species