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Immunohistochemistry revealed the presence of VIP-, NPY- and  SP-immunoreactivity in the rat adrenal medulla. VIP- and NPY-immunoreactivity  was detected in chromaffin and ganglion cells and in nerve fibers, but  SP-immunoreactivity was found only in chromaffin cells. After acute heat  stress, VIP- and NPY- immunoreactivities in cells and nerve fibers were  reduced, probably as a result of the release of these peptides with  catecholamines. The absence of SP-immunoreactive ganglion cells in the  adrenal medulla suggests that the SP-immunoreactive nerve fibers are  extrinsic in origin

archives of biological sciences

Immunohistochemical evidence for the presence of a vasoactive intestinal peptide, neuropeptide Y and substance P in rat adrenal medulla after heat stress