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The aim of this work was to examine the impact of urban wastewaters on the  water quality of the Danube River in Serbia. Samples of water and sediments  for microbiological analysis and genotoxicity monitoring were collected from  6 sites during spring and/or autumn 2010. Sanitary analysis, i.e. enumeration  of total and fecal coliforms and intestinal enterococci, indicated moderate  to critical fecal contamination, while organic load assessment (oligotroph to  heterotroph ratio, index of phosphatase activity) revealed the category of  moderately polluted water. Mercury-resistant bacteria were detected in all  water samples, with high numbers at locations positioned downstream of  Belgrade. There was no correlation of the microbiological parameters of the  sediment and water samples. Genotoxicity monitoring, performed by the comet  assay on hemocytes of mussels Sinanodonta woodiana, indicated a significant  increase of DNA damage in mussels collected from the studied sites compared  with the control group

The anthropogenic impact on water quality of the river Danube in Serbia: Microbiological analysis and genotoxicity monitoring