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The antifungal activity of Nepeta rtanjensis Diklić & Milojević essential oil  was tested against the human pathogenic fungus Bipolaris spicifera (Bainier)  Subramanian via mycelial growth assay and conidia germination assay. The  minimally inhibitory concentration (MIC) of the oil was determined at 1.0 μg  ml-1, while the MIC for the antifungal drug Bifonazole in a positive control  was determined at 10.0 μg ml-1. The maximum of conidia germination inhibition  was accomplished at 0.6 μg ml-1. In addition, at 0.6 μg ml-1 and 0.8 μg ml-1  the oil was able to cause morphophysiological changes in B. spicifera. The  most significant result is the bleaching effect of the melanized conidial  apparatus of the test fungi, since the melanin is the virulence factor in  human pathogenic fungi. These results showed the strong antifungal properties  of N. rtanjensis essential oil, supporting its possible rational use as an  alternative source of new antifungal compounds

In vitro antifungal and demelanizing activity of Nepeta rtanjensis essential oil against the human pathogen Bipolaris spicifera