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An acid phosphatase has been isolated and purified from an extract of a  germinating black gram seedling. The method was accomplished by gel  filtration of a germinating black gram seedling crude extract on sephadex  G-75 followed by ion exchange chromatography on DEAE cellulose. The acid  phosphatase gave a single band on SDS-polyacrylamide slab gel  electrophoresis. The molecular weight of the acid phosphatase determined by  SDS-polyacrylamide slab gel electrophoresis was estimated to be 25 kDa. The  purified enzyme showed maximum activity at pH 5 and at temperature of 55°C.  Mg2+, Zn2+ and EDTA had an inhibitory effect on the activity of the acid  phosphatase. Black gram seedling acid phosphatase was activated by K+, Cu2+  and Ba2+. The Km value of the enzyme was found to be 0.49 mM for pNPP as  substrate

Purification and characterization of acid phosphatase from a germinating black gram (Vigna mungo L.) seedling