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Diverse protocols have been used in the shrimp industry to produce  microalgae. In this work, we evaluated the effect of inoculation time (0600  and 1200) and initial microalgae density (0.4 and 0.8 x105cells mL-1) in  static outdoor cultures of Tetraselmis chuii, using F/2 and 2-F media. A  significant effect on cell density (> 6.7 x 105 cells mL-1) was found after  treatments with a high initial density and in the F/2 medium. The higher  productive responses were observed in treatments inoculated at 0600. The  better productive response was found in treatments inoculated at 0600 with  0.8 x 105 cells•mL-1 in the F/2 medium

archives of biological sciences

The effect of inoculation time and inoculum concentration on the productive response of Tetraselmis chuii (Butcher, 1958) mass cultured in F/2 and 2-F media