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We analyzed 93 couples undergoing male screening with the Sperm Penetration  Assay (SPA) before in vitro fertilization and intracytoplasmic sperm  injection (ICSI), to determine the accuracy of SPA for subsequent embryonic  development, incidence of pregnancy and miscarriage rates (SAB). ICSI  patients with the lowest SPA scores had significantly higher incidences of  Sthan did patients in the other SPA groups. Sperm quality is higher with  better SPA scores. Poor sperm quality has increased incidence of chromosomal  abnormalities and is associated with early fetal loss. Couples with negative  SPA are candidates for preimplantation genetic diagnosis, to reduce the  incidence of SAB

archives of biological sciences

Correlation of the sperm penetration assay (SPA) and miscarriage after assisted reproduction: The potential use of spa as a new criterion for preimplantation genetic diagnosis