Bacteriophage λ proliferation in Escherichia coli under the influence of microwave irradiation
Author(s) -
Nataša Milojević,
Dragomir Stanisavljev,
Biljaikolić,
Miloš V. Beljanski,
Ljiljana Kolar-Anić,
Jelena KneževićVukčević
Publication year - 2010
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs1004935m
Subject(s) - bacteriophage , escherichia coli , microwave , microwave irradiation , absorption (acoustics) , kinetics , irradiation , chemistry , adsorption , bacterial growth , microbiology and biotechnology , bacteria , biophysics , biology , materials science , biochemistry , physics , genetics , quantum mechanics , nuclear physics , composite material , gene
The influence of microwaves on bacterial metabolism was investigated using the proliferation of bacteriophage λ in Escherichia coli cells as a model system. All experiments were performed under the same microwave absorption rate and constant temperature. Microwave treatment had no effect on bacterial or phage viability, or on phage adsorption. Microwaves significantly influenced phage proliferation but the effects depended on the experimental temperature. The kinetics of phage proliferation decreased with irradiation at the optimal temperature and increased at the suboptimal temperature. This result could be ascribed to the specific thermal effects of microwaves.
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