Morphological and molecular identification of potato cyst nematode populations in Serbia | Zendy

Violeta Oro | Zendy; Žarko Ivanović | Zendy; Bogdan Nikolić | Zendy; L. Barsi | Zendy; Milan Radivojević | Zendy; Branko Jovčić | Zendy

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Morphological and molecular identification of potato cyst nematode populations in Serbia

Author(s) -

Violeta Oro,

Žarko Ivanović,

Bogdan Nikolić,

L. Barsi,

Milan Radivojević,

Branko Jovčić

Publication year - 2010

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs1003747o

Subject(s) - biology , potato cyst nematode , globodera pallida , globodera rostochiensis , dna extraction , identification (biology) , nematode , dna barcoding , specific identification , morphometrics , quarantine , botany , isolation (microbiology) , zoology , ecology , polymerase chain reaction , genetics , solanaceae , microbiology and biotechnology , gene

Quarantine species such as potato cyst nematodes Globodera rostochiensis and G. pallida are present in Serbia since 1999 and 2005, respectively. These nematodes are sibling species and their morphological identification is complex due to their morphometric overlap. The cysts from the localities of Kladnica, Šanac, Gojna Gora and Milatovići were grown on susceptible potato varieties and their morphological differences have been discussed. To avoid ambiguities in species morphological designation a duplex PCR method was chosen for a rapid and accurate species identification. The whole procedure, from DNA extraction to DNA isolation, can be performed in a single day.

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