Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes | Zendy

Ana Bratić | Zendy; Dragana Majic | Zendy; Jelena Samardžić | Zendy; Martin Kragl | Zendy; Vesna Maksimović | Zendy

Open Access

Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes

Author(s) -

Ana Bratić,

Dragana Majic,

Jelena Samardžić,

Martin Kragl,

Vesna Maksimović

Publication year - 2010

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs1001057b

Subject(s) - gene , biology , promoter , promoter activity , gene expression , regulatory sequence , pollen , transcription (linguistics) , genetics , microbiology and biotechnology , computational biology , botany , philosophy , linguistics

Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in both systems

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