Characterization of antibodies directed against the Ankrd2 human muscle protein | Zendy

Snežana Kojić | Zendy; Elisa Medeot | Zendy; Georgine Faulkner | Zendy

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Characterization of antibodies directed against the Ankrd2 human muscle protein

Author(s) -

Snežana Kojić,

Elisa Medeot,

Georgine Faulkner

Publication year - 2009

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs0904683k

Subject(s) - epitope , polyclonal antibodies , monoclonal antibody , recombinant dna , antibody , microbiology and biotechnology , epitope mapping , linear epitope , biology , biochemistry , immunology , gene

In order to study the function of the Ankrd2 protein, for which commercial antibodies are not available, we report the production and analysis of polyclonal antibodies to full-length Ankrd2 and its C-terminal and N-terminal regions, as well as a monoclonal antibody to the C-terminus of the protein. Epitope mapping making use of recombinant deletion mutants showed that an epitope located in region 323-333 aa of Ankrd2 is detected by the monoclonal antibody. The high specificity of all four anti-Ankrd2 antibodies for recombinant and endogenous Ankrd2 protein is also demonstrated

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