RAPD analysis of genetic diversity and qualitative assessment of hydrolytic activities in a collection of Bacillus sp. isolate | Zendy

Tanja Berić | Zendy; María C. Urdaci | Zendy; Slaviša Stanković | Zendy; Jelena KneževićVukčević | Zendy

Open Access

RAPD analysis of genetic diversity and qualitative assessment of hydrolytic activities in a collection of Bacillus sp. isolate

Author(s) -

Tanja Berić,

María C. Urdaci,

Slaviša Stanković,

Jelena KneževićVukčević

Publication year - 2009

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs0904645b

Subject(s) - rapd , biology , cereus , genetic diversity , amylase , bacillus cereus , bacillus subtilis , enzyme , lipase , food science , botany , microbiology and biotechnology , bacteria , biochemistry , genetics , population , demography , sociology

Genetic diversity and production of hydrolytic enzymes of 205 Bacillus isolates from different geographical and ecological niches in Serbia were studied. Combining RAPD analysis and 16S DNA sequencing, we determined 13 different groups of RAPD profiles within four (five) species: B. subtilis, B. cereus/B. thuringiensis, B. pumilus, and B. firmus. Screening for production of hydrolytic enzymes showed that there was no correlation of enzyme production with species. Most of the isolates from all habitats produced amylase, protease, lipase, mannanase, and xylanase to some extent at 25ºC and 37ºC. The number of isolates that retained enzyme production ability at 55ºC is considerably lower and they predominantly came from manure

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