In HCMV-exposed HUVEC, P52/RelB regulatory factors mediate activation of the human PAR1 gene promoter | Zendy

Miroslav Popović | Zendy; Svetlana Paskaš | Zendy; Maja Živković | Zendy; Tamara Djuric-Delic | Zendy; Aleksandra Stanković | Zendy; Ladislav Burýšek | Zendy

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In HCMV-exposed HUVEC, P52/RelB regulatory factors mediate activation of the human PAR1 gene promoter

Author(s) -

Miroslav Popović,

Svetlana Paskaš,

Maja Živković,

Tamara Djuric-Delic,

Aleksandra Stanković,

Ladislav Burýšek

Publication year - 2009

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs0904613p

Subject(s) - relb , reporter gene , transfection , promoter activity , jurkat cells , microbiology and biotechnology , gene , chemistry , biology , promoter , gene expression , transcription factor , t cell , nfkb1 , immunology , genetics , immune system

We studied the effect of HCMV (AD-169) on the regulation of PAR1 reporter activity in HUVEC. In HUVEC, HCMV significantly induced activation of PAR1 reporter constructs carrying AP-2-like and two complexes of AP-2/Sp1-binding elements. Shorter constructs with no AP-2-like binding elements had lower activity upon virus inoculation. Moreover, activity of the shortest construct without binding elements was not significantly increased in virus-exposed cells. Furthermore, in HCMV-exposed cells, activity of the PAR1 reporter was significantly augmented upon p52/RelB transfection. Taken together, the data indicate that activity of the PAR1 promoter is highly dependent on AP-2/Sp1 binding elements and mediated by p52/RelB regulatory factors. Key words: HCMV, PAR1 gene reporter, HUVEC

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