Efficient genetic transformation of Impatiens hawkerii Bull. (Balsamiaceae) using agrobacterium rhizogenes | Zendy

Snežana Milošević | Zendy; Angelina Subotić | Zendy; Aleksandar Cingel | Zendy; Slađana Jevremović | Zendy; Slavica Ninković﻿﻿﻿ | Zendy

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Efficient genetic transformation of Impatiens hawkerii Bull. (Balsamiaceae) using agrobacterium rhizogenes

Author(s) -

Snežana Milošević,

Angelina Subotić,

Aleksandar Cingel,

Slađana Jevremović,

Slavica Ninković

Publication year - 2009

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs0903467m

Subject(s) - agrobacterium , biology , clone (java method) , transformation (genetics) , botany , explant culture , inoculation , shoot , hairy root culture , horticulture , genetics , gene , in vitro

Transformation of Impatiens hawkerii Bull. mediated by Agrobacterium rhizogenes strain A4M70GUS was studied. Hairy roots developed 10 days after inoculation were excised from the shoot explants and transferred onto Murashige and Skoog's (MS) basal medium lacking plant growth regulators. More than 20 hairy root clones were established and eight of them were further analyzed. Each clone differed significantly from the others in growth capacity and lateral branching. Clone C2 showed the highest biomass (20.6 g L-1) as well as the highest number of lateral roots (37 ± 2.2). The transgenic nature of the established hairy root clones was confirmed by GUS assay and PCR analysis. In conclusion, hairy roots were developed for the first time in I. hawkerii Bull., and transgenic hairy root clones showed a distinct morphological nature and growth patterns

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