Plant regeneration of southern Adriatic iris by somatic embryogenesis | Zendy

Slađana Jevremović | Zendy; Angelina Subotić | Zendy; Milana TrifunovićMomčilov | Zendy; Marija Nikolic | Zendy

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Plant regeneration of southern Adriatic iris by somatic embryogenesis

Author(s) -

Slađana Jevremović,

Angelina Subotić,

Milana TrifunovićMomčilov,

Marija Nikolic

Publication year - 2009

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs0903413j

Subject(s) - somatic embryogenesis , biology , somatic cell , ploidy , embryo , botany , regeneration (biology) , germination , embryogenesis , microbiology and biotechnology , genetics , gene

A simple protocol has been developed for plant regeneration by somatic embryogenesis of Southern Adriatic iris (Iris pseudopallida Trinajstić), an endemic species of the Balkan Peninsula. Somatic embryogenesis was induced in zygotic embryo culture on media supplemented with 2,4-dichlorophenoxy acetic acid (2-10 mgL-1) as the sole plant growth regulator, where both embryogenic calli and somatic embryos were induced. Subsequent decrease of 2,4-D in the media promoted formation of somatic embryos. Developed somatic embryos germinated on medium without growth regulators. The regenerated plantlets had diploid chromosome number. Planted plantlets acclimatized very well under greenhouse and garden conditions

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