Open AccessLight modulates activity and expression of glutamine synthetase isoforms in maize seedling roots
Author(s) -
Ana Simonović,
Marc D. Anderson
Publication year - 2008
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs0804649s
Subject(s) - darkness , glutamine synthetase , gene isoform , seedling , biology , cytosol , glutamine , transcription (linguistics) , hypocotyl , botany , biochemistry , enzyme , microbiology and biotechnology , gene , linguistics , philosophy , amino acid
In maize roots, continuous illumination inhibits chloroplastic glutamine synthetase (GS2) activity, which decreased in light from 72.8% in 4-day-old to 26% in 10-day-old plants. In dark-adapted plants transferred to light for 6 days, GS2 activity declined from 100% to 41%, but in light-adapted plants transferred to darkness, it increased to the level of the dark control. Changes of cytosolic (GS1) activity were minor, with a similar trend. Quantitative RT-PCR revealed that light/dark treatments moderately affected only transcription of GS1 isoforms, with the exception of GS1-2, which was dramatically induced by darkness and repressed by light
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