Establishing the resistance of Myzus persicae (Sulzer) by molecular methods | Zendy

Andja Vučetić | Zendy; Olivera Petrović-Obradović | Zendy; John T. Margaritopoulos | Zendy; Panagiotis J. Skouras | Zendy

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Establishing the resistance of Myzus persicae (Sulzer) by molecular methods

Author(s) -

Andja Vučetić,

Olivera Petrović-Obradović,

John T. Margaritopoulos,

Panagiotis J. Skouras

Publication year - 2008

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs0803493v

Subject(s) - myzus persicae , aphididae , aphid , biology , esterase , insecticide resistance , gene , horticulture , resistance (ecology) , genetics , botany , pest analysis , toxicology , homoptera , agronomy , enzyme , biochemistry

In two years of investigating resistance of the peach-potato aphid Myzus persicae (Sulzer) by molecular methods, several types of resistance were established in the majority of individuals from peach and tobacco in Serbia and Montenegro. Most of the tested individuals had the FE4 gene, which encodes production of FE4 esterase. The gene responsible for kdr (knock-down resistance) was found in the majority of individuals, but in the heterozygous state, while resistance based on formation of modified acetlycholinesterase (MACE) was least represented. Also, tests showed aphids from tobacco to be more sensitive to insecticide action than aphids from peach. Three tests were used in these investigations, e.g., the PCR - esterase, PCR - kdr, and RFLP - PCR tests, each for a single type of resistance

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