Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae | Zendy

Nataša Božić | Zendy; Biljana Dojnov | Zendy; Aleksandra Milovanović | Zendy; Vera Nenadović | Zendy; Jelisaveta Ivanović | Zendy; Zoran Vujčić | Zendy

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Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae

Author(s) -

Nataša Božić,

Biljana Dojnov,

Aleksandra Milovanović,

Vera Nenadović,

Jelisaveta Ivanović,

Zoran Vujčić

Publication year - 2008

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs0803403b

Subject(s) - midgut , biochemistry , zymography , serine , chromogenic , trypsin , chemistry , biology , microbiology and biotechnology , enzyme , larva , chromatography , botany

Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities

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