Post-translational regulation of the RpoS and PsrA genes in pseudomonas putida WCS358: The role of ClpXP protease | Zendy

Branko Jovčić | Zendy; Jelena Begović | Zendy; Jelena Lozo | Zendy; L. Topisirović | Zendy; Milan Kojić | Zendy

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Post-translational regulation of the RpoS and PsrA genes in pseudomonas putida WCS358: The role of ClpXP protease

Author(s) -

Branko Jovčić,

Jelena Begović,

Jelena Lozo,

L. Topisirović,

Milan Kojić

Publication year - 2008

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs0801001j

Subject(s) - rpos , pseudomonas putida , biology , protease , sigma factor , serine protease , pseudomonas , rpon , gene , microbiology and biotechnology , enzyme , biochemistry , bacteria , genetics , escherichia coli , gene expression , promoter , rna polymerase

The RpoS and PsrA proteins are key transcriptional regulators that are activated in response to the stationary phase of growth in pseudomonads. This study was designed to establish whether ClpXP (ATP-dependent serine protease) regulates levels of RpoS and PsrA in Pseudomonas putida WCS358. Western blot analysis of P. putida WCS358 protein extracts from the early exponentianl, late exponential, and stationary phases of growth with antibodies against RpoS and PsrA revealed that these proteins are degraded by ClpXP in the early exponential phase of growth. The obtained results demonstrate a role for ClpXP protease in post-translational regulation of proteins encoded by the rpoS and psrA genes in Pseudomonas spp

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