In planta transformation of buckwheat (Fagopyrum esculentum Moench.)
Author(s) -
Ana Bratić,
Dragana Majic,
J. MiljušDjukič,
Živko Jovanović,
Vesna Maksimović
Publication year - 2007
Publication title -
archives of biological sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.217
H-Index - 25
eISSN - 1821-4339
pISSN - 0354-4664
DOI - 10.2298/abs0702135b
Subject(s) - fagopyrum , infiltration (hvac) , transformation (genetics) , biology , botany , gene , materials science , genetics , composite material
In order to develop a reliable and rapid transgenic system for functional study of specific buckwheat gene constructs, two different in planta transformation methods were analyzed: vacuum infiltration and infiltration by syringe. The results indicated that the vacuum infiltration method was much more efficient and can therefore be considered the method of choice for buckwheat transformation.
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