Development of the moss Pogonatum urnigerum (Hedw.) P. Beauv. under in vitro culture conditions | Zendy

Tijana Cvetić Antić | Zendy; Aneta Sabovljević | Zendy; Marko Sabovljević | Zendy; D. Grubišić | Zendy

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Development of the moss Pogonatum urnigerum (Hedw.) P. Beauv. under in vitro culture conditions

Author(s) -

Tijana Cvetić Antić,

Aneta Sabovljević,

Marko Sabovljević,

D. Grubišić

Publication year - 2007

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs0701057c

Subject(s) - protonema , gametophyte , moss , callus , botany , spore , sucrose , cytokinin , auxin , biology , in vitro , tissue culture , nutrient , horticulture , food science , biochemistry , ecology , pollen , gene

Pogonatum urnigerum (Polytrichaceae) in vitro culture was established from spores collected in nature. Both protonema and gametophore stages of gametophyte development were obtained. Also, a stable callus culture was established using hormone-free nutrient medium. The best nutrient medium for development was half-strength Murashige- Skoog medium supplemented with 1.5% sucrose. Auxin treatment enabled some gametophores to develop, but prolonged treatment induced early senescence. Tissues grown on cytokinin did not produce any gametophytes and did not survive prolonged treatment

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