Treatment of isolated pistils with protease inhibitors overcomes the self-incompatibility response in buckwheat | Zendy

J. MiljušDjukič | Zendy; Svetlana Radović | Zendy; Vesna Maksimović | Zendy

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Treatment of isolated pistils with protease inhibitors overcomes the self-incompatibility response in buckwheat

Author(s) -

J. MiljušDjukič,

Svetlana Radović,

Vesna Maksimović

Publication year - 2007

Publication title -

archives of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.217

H-Index - 25

eISSN - 1821-4339

pISSN - 0354-4664

DOI - 10.2298/abs0701045m

Subject(s) - pepstatin , pollen tube , gynoecium , proteases , protease , antipain , biology , pollen , botany , fagopyrum , protease inhibitor (pharmacology) , biochemistry , leupeptin , enzyme , pollination , immunology , human immunodeficiency virus (hiv) , stamen , antiretroviral therapy , viral load

Isolated pistils of distylous buckwheat (Fagopyrum esculentum Moench) were treated with protease inhibitors (PMSF, pepstatin A, and antipain). Pistils were cross- or self- pollinated, and growth of pollen tubes was observed under a fluorescence microscope. Treatments with all inhibitors suppressed inhibition of self-pollen tube growth, suggesting that activity of proteases is involved in rejection of self-pollen during the SI response

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