Influence of permeabilization on the catalytic activity of yeast cells Yarrowia lipolytica kkp 379 in the synthesis of plasticizer – dioctyl adipate

Plasticizers are among the most important additives required for processing of polymer materials. In this paper, biotechnological synthesis of adipic plasticizer – dioctyl adipate was presented with the participation of yeast biomass of Yarrowia lipolytica KKP 379. The reaction was based on transesterification of dimethyl adipate with octan-1-ol. Relative to the whole-cell catalyst were used both physical (treated with glass beads) and chemical (use of isooctane and Triton X-100) methods of permeabilization. The research confirmed the possibility of the synthesis of dioctyl adipate catalyzed by whole cell yeast with the yield 60% (58% ±1,8%). The permeabilization techniques used did not contribute to increasing the reaction efficiency. Among the methods of violating the continuity of the yeast cell wall, the chemical method using Triton X-100 is of interest. Approximately 50% conversion was achieved in this reaction and no decrease in yield was observed during 96 hours of reaction, as opposed to other methods permeabilization.