Efecto de la modulación del receptor nuclear PPAR en la diferenciación de células de preadipocitos de fibroblastos

Adipogenesis from preadypocites of mouse fibroblasts is evaluated by using Roziglitazone and Insulin. To that effect, mouse preadipocytes of the cellular line 3T3-L1 are incubated in a differentiation cocktail with Rosiglitazone and Insulin. Observations are made between 0 and 10 days with a frequency of two days. The adipocytes differentiation process was valued through the amount of present triglycerides from the fatty cells using qualitative methods by dying and quantitative methods from the extraction of fatty acids with isopropanol and measuring absorbance at 510 nm. The results obtained allowed us to confirm the adipogenic from Rosiglitazone and from Insulin, althoughboth showed differences in their adipogenic ability because Rosiglitazone is a synthetic PPARa nuclear receptor ligand. When activated, it induces the expression of the genes involved in the adipogenesis process while insulin acts on the membrane receptor for the growth factor insulin type (IGF-1) which route does not activate the PPARa nuclear receptor directly resulting in an adipogenic process slower when compared to Rosiglitazone. These results will allow us to develop new medicines in the future for the treatment of diseases such as obesity and type-2 diabetes.