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strains of the family EnterobacteriaceaeandPseudomonadaceaewere conserved by filter paper semistock method and 68 strains by Cryopreservation stock method in the bank of strains and genes of Biotechnology Institute of the Universidad Nacional de Colombia (IBUN). The effectiveness of methods was tested for the viability at 24 hours after preservation and was calculated the recovery percentage. Thus in conclusion, the preservation in paper filter and cryopreservation are suitable semistock and Stock methods, respectively; because 73.7% of familyEnterobacteriaceaemicroorganisms and 82.3% of familyPseudomonadaceaemicroorganisms, presented a high recovery percentage greater 50%. In addition, was implemented a strain bank data base made in software Access ®, in Biotechnology Institute and Universidad Nacional de Colombia Web page www.ibun.unal.edu.co/instituto/cepario. It is available for general public.

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Implementación y evaluación de dos métodos deconservación y generación de la base de datos del banco de cepas y genes del Instituto de Biotecnología de la Universidad Nacional de Colombia (IBUN)