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Influence of a-Factor over the expression of hrIDS in Pichia pastoris: Literature review and computational analysis. In searching of alternatives to get the efficient secretions of the recombinant enzyme Iduronate Sulfatase (IDSh) in Pichia pastoris, we made a systematic review of the literature so that we could compile information to relate the expression level of human heterologous proteins, with secretion signal, and the characteristics of the molecules. 349 publications were found of which only 7 (2%) reported the expression of proteins that met find inclusion criteria. With the information obtained in these articles, we made a hypothesis test using the data compiled as samples and a qualitative analysis of the information. It was found that replacement of native secretion signal bya-Factor as leader peptide increases the level of expression of human heterologous

Influencia del a-Factor sobre la expresión de IDShr en Pichia pastoris: Revisión sistemática de literatura y análisis computacional