Spatially varying deconvolution for light-sheet microscopy | Zendy

Bogdan Toader | Zendy

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Spatially varying deconvolution for light-sheet microscopy

Author(s) -

Bogdan Toader

Publication year - 2020

Language(s) - English

Resource type - Conference proceedings

DOI - 10.22443/rms.lsfm2020.44

Subject(s) - deconvolution , light sheet fluorescence microscopy , microscopy , optical microscope , optics , materials science , computer science , computer graphics (images) , physics , scanning confocal electron microscopy , scanning electron microscope

Light-sheet microscopy is a type of fluorescence microscopy used in cell biology due to its fast acquisition times and low photo-damage to the sample. This is achieved by selectively illuminating a slice of the sample using a sheet of light and detecting the emitted fluorescence signal using a dedicated objective orthogonal to the plane of the sheet. Consequently, the effective point spread function (PSF) of the microscope is spatially varying, which adds an additional layer of complexity to the problem of deconvolution of such images.

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