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A System-level Approach to Single-Molecule Live-Cell Fluorescence Microscopy
Author(s) -
Oliver Harriman,
Mark C. Leake
Publication year - 2013
Publication title -
infocus magazine
Language(s) - English
Resource type - Journals
ISSN - 1750-4740
DOI - 10.22443/rms.inf.1.93
Subject(s) - fluorescence microscope , fluorescence , microscopy , molecule , biophysics , single molecule experiment , nanotechnology , chemistry , materials science , optics , physics , biology , organic chemistry
5 A system-level Approach to single-Molecule Live-Cell Fluorescence Microscopy Single-molecule fluorescence microscopy provides a window into the molecular activity of the living cell. Through genetically fusing molecules of interest with fluorescent proteins, specific biological mechanisms can be elucidated. While the approach is maturing rapidly with the application of numerous inventive optical and analytical techniques, it is still a challenge to generate adequate imaging conditions.

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