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A simple, rapid, accurate, precise, specific and economical spectrophotometric method was used for determination of phenytoin .In this study, a second derivative spectrophotometric method was used for the determination of phenytoin (PHT). This method obeys Beer’s law in the employed concentration ranges of 2-10 ppm for phenytoin. The UV derivative spectra D for (PHT) have (V) at 233.3 nm,D spectra for (PHT) have (P) at 226.7 nm, D spectra for PHT) have (P) at 223.6 nm, and (V) at 230.4 nmD spectra for (PHT) have (P) at 236.5 nm, and (V) at 228.5nm.thelinear concentration ranges (2-10) ppm with correlation coefficient (r) 0.9996, 0.9987, 0.9973, 0.9979. The second derivative D tech. at (226.7) nm successfully applied for determination the Phenytoin drug in pharmaceutical samples. Results of analysis were validated statistically and by recovery studies.

Second Derivative Spectrophotometric Determination of Phenytoin in Pharmaceutical Preparations