VITAMINS PROVOKE THE RELEASE OF NITRIC OXIDE BY SODIUM NITROPRUSSIDE: IN VITRO STUDY

It is plausible to assume that vitamins act as nitric oxide scavengers or donors like cobalamine derivatives. Therefore, this study aimed to screen the effect of folic acid, hydroxycobalamine and phytomenadione on nitrogen species. The effects of hydroxyl cobalamine, folic acid and phytomenadione were studied on the synthesized peroxynitrite as well as on their ability to generate peroxynitrite. It is ability to donate nitric oxide or to scavenge released nitric oxide by sodium nitroprusside (10mM) also investigated in vitro experimental model. Synthetic peroxynitrite was scavenged by folic acid, hydroxycobalamine and phytomenadione. Their effects varied with their concentrations. These vitamins per se failed to release nitric oxide while they improved the bioavailability of nitric oxide released by 10 mM sodium nitroprusside. The beneficial effects of folic acid and to less extent hydroxycobalamine and phytomenadione in improving the nitric oxide bioavailability raise the idea of using these substances as adjunct therapy in diseases associated with endothelial dysfunction.