Maternal plazmadaki hücre dışı serbest fetal DNA kullanılarak girişimsel olmayan fetal RhD genotiplemesinin tanısal doğruluğu

The non-invasive prenataldiagnosis of the fetus RhD genotype in RhD incompatibility has a crucial rolein the prevention of increased anti-D immunoglobulin therapy for haemolyticdiseases in pregnant women carrying RhD negative fetus. It was aimed to detectfetal RhD genotyping by using maternal circulating cell-free DNA in the currentstudy.Methods: Maternal blood samples werecollected in different trimester of pregnancies (12-40 weeks) in 12 D-negativemothers. Cell-free fetal DNA was extracted from 2 ml of maternal plasma by anconventional DNA isolation technique (Qiagen, Hilden, Germany) and real-timePCR was performed for genotyping target RhD exons 7 and 10 and GLO genes.Postnatal serological evaluations were performed and the results wereconfirmed.Results: 6 cases (50 %)were determined D positive and 6 cases (50 %) were determined D negative. Allresults were also confirmed after birth serologically.Conclusions: In conclusion,the current results showed us the non-invasive target RhD genotyping from cellfree fetal DNA from maternal plasma samples have a diagnostic accuracy in RhDincompatibility pregnancies.