GC-MS ANALYSIS OF FIXED OILS OF NIGELLA SATIVA SEEDS | Zendy

Sania Feroz | Zendy

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GC-MS ANALYSIS OF FIXED OILS OF NIGELLA SATIVA SEEDS

Author(s) -

Sania Feroz

Publication year - 2018

Publication title -

universal journal of pharmaceutical research

Language(s) - English

Resource type - Journals

ISSN - 2456-8058

DOI - 10.22270/ujpr.v3i2.139

Subject(s) - nigella sativa , palmitic acid , oleic acid , chemistry , chromatography , linoleic acid , gas chromatography–mass spectrometry , mass spectrometry , traditional medicine , fatty acid , medicine , organic chemistry , biochemistry

Gas chromatography-Mass spectrometry (GC-MS) is an analytical method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, fire investigation, environmental analysis, explosives investigation, and identification of unknown samples. The present study also relies on use of GC-MS for detection and interpretation of compounds present in N. sativa oil samples. Fixed oil was obtained through column chromatography of ethyl acetate fraction. The oil samples were subjected to GC-MS analysis which showed 5, 18, 12 and 20 compounds in four fixed oil samples respectively. The major components were linoleic acid, methyl ester (35.5%), oleic acid, methyl ester (15.007%), palmitic acid, methyl ester (8.208%).

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