Isolation of Actinomycetes Strains and their Different Levels of Resistance against Antibiotics | Zendy

Khwaja Salahuddin | Zendy; Md. Nizamuddin Ansari | Zendy; Chandan Kumar Singh | Zendy; Anupama Anupama | Zendy; Amit Kumar Jha | Zendy; Ritisha N. Tatmiya | Zendy; Syeda Fauzia Farheen Zofair | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Isolation of Actinomycetes Strains and their Different Levels of Resistance against Antibiotics

Author(s) -

Khwaja Salahuddin,

Md. Nizamuddin Ansari,

Chandan Kumar Singh,

Anupama Anupama,

Amit Kumar Jha,

Ritisha N. Tatmiya,

Syeda Fauzia Farheen Zofair

Publication year - 2020

Publication title -

journal of pure and applied microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.149

H-Index - 16

eISSN - 2581-690X

pISSN - 0973-7510

DOI - 10.22207/jpam.14.2.47

Subject(s) - isolation (microbiology) , antibiotics , microbiology and biotechnology , biology , resistance (ecology) , ecology

This study is aimed at isolating and characterizing strains of an actinomycetes from soils. Six strains of actinomycetes were isolated from the riverside, rhizoplane and low land of Muzaffarpur district of Bihar (India). The cultural characterisation revealed the production of diffusible pigments of different colours. Heavy sporulation was noticed in some strains. Out of six strains isolated, four strains such as KS-1, KS-2, KS-3 and KS-4 were found to be mesophilic, and the other two KS-5 and KS-6 showed thermophilic when subjected to a higher temperature. The antibiotic susceptibilities of these six strains gave the idea about the sensitive and resistance nature based on the zone of inhibition against six antibiotics. Three strains (KS-1, KS-3 and KS-5) were found to be sensitive, whereas KS-2, KS-4 and KS-6 exhibited resistance to the particular antibiotic. The antibiogram of these strains such as KS-2, KS-4 and KS-6 clearly showed the different levels of resistance such as constitutive resistance, lethal dose50 and minimal inhibitory concentration within the susceptible range of different antibiotics. CR was found to be maximum (3.5 μgmL-1) in strain KS-4 against tetracycline, the highest lethal dose (14μgmL-1) was noticed in KS-2 against ampicillin while Minimal Inhibitory concentration was found to be maximum (26μgmL-1) against streptomycin in strain KS-6.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research