Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6 | Zendy

Kunal Kumar | Zendy; Prasanna D. Belur | Zendy

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Production of Oxalate Oxidase from Endophytic Ochrobactrum intermedium CL6

Author(s) -

Kunal Kumar,

Prasanna D. Belur

Publication year - 2018

Publication title -

journal of pure and applied microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.149

H-Index - 16

eISSN - 2581-690X

pISSN - 0973-7510

DOI - 10.22207/jpam.12.4.75

Subject(s) - oxalate , chemistry , organic chemistry

Four oxalate degrading endophytic bacteria were isolated from oxalate rich Colocasia esculenta tubers. Based upon the oxalate oxidase (EC 1.2.3.4) activity produced in nutrient medium, one bacterium was selected and identified as Ochrobactrum intermedium by 16S rDNA sequencing. Studies on effect of nutritional and non-nutritional parameters showed that oxalate oxidase production is inducible, requires Manganese ions in the medium, and very low fill-up volume is beneficial. Shake flask fermentation carried out with medium comprising Sucrose, Ammonium chloride, Sodium oxalate along with basal salts gave 0.5 UmL-1 oxalate oxidase activity and 0.454 Umg-1specific activity after 65h of fermentation.

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