Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes | Zendy

Raghavendrarao Sanagala | Zendy; Ranjitha Kumari B.D | Zendy

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Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes

Author(s) -

Raghavendrarao Sanagala,

Ranjitha Kumari B.D

Publication year - 2018

Publication title -

journal of pure and applied microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.149

H-Index - 16

eISSN - 2581-690X

pISSN - 0973-7510

DOI - 10.22207/jpam.12.4.54

Subject(s) - regeneration (biology) , microrna , biology , genotype , botany , microbiology and biotechnology , horticulture , gene , genetics

A highly efficient and reproducible tissue culture regeneration system is an important prerequisite for achieving successful transformation. However, most indica rice varieties remain recalcitrant to genetic transformation and require refinement of the tissue culture protocol for the generation of embryogenic calli or regeneration of fertile plants from transformed cells. In this study, standardized regeneration system for indica rice cultivars was carried out. Further the expression of miR164c was investigated in the different rice genotypes. The expression of miR164c was proportional to the percentage of regeneration of the rice genotypes. These results provide interesting insights that will significantly improve our understanding of direct regeneration of rice from callus tissue.

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